Affinty Tag

Review

TRENDS in Biotechnology

Vol.23 No.6 June 2005

Making the almost of af?nity tags
David S. Waugh
Protein Engineering Section, Macromolecular Crystallography Laboratory, condense for Cancer Research, National Cancer Institute at Frederick, P.O. misfortune B, Frederick, MD 21702-1201, USA

Proteins do not naturally lend themselves to highthroughput synopsis because of their diverse physiochemical properties. Consequently, af?nity tags have become indispensable tools for structural and useful proteomics initiatives. Although originally developed to facilitate the detection and puri?cation of recombinant proteins, in upstart years it has become clear that af?nity tags bathroom have a positive impact on the yield, solubility and even the folding of their fusion partners. However, no single af?nity tag is optimal with respectfulness to all of these parameters; each has its strengths and weaknesses. Therefore, combinatorial tagging might be the and way to harness the in full potential of af?nity tags in a high-throughput setting. Introduction As we enter the post-genomic era, the focus is shifting from high-throughput outline of genome sequences to functional and structural studies of the proteins they encode.



However, proteins argon much more chemically and structurally diverse than nucleic acids, making them intrinsically unsuitable for generic wine methodology. As a result, the use of genetically engineered af?nity tags is the only instrument of achieving high-throughput protein puri?cation. The variable yield and poor solubility of many recombinant proteins are also major impediments to highthroughput production. However, the yield of recombinant proteins can often be improved by the judicious use of af?nity tags and some tags can enhance the solubility, and even promote the proper folding, of their fusion partners. No single tag is ideally suited for all of these purposes. Instead, dickens or more tags are needed to make the most of af?nity tagging in a high-throughput setting. To...If you want to get a full essay, order it on our website: Orderessay



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